Journal: Journal of Extracellular Vesicles
Article Title: CD73 + extracellular vesicles inhibit angiogenesis through adenosine A 2B receptor signalling
doi: 10.1080/20013078.2020.1757900
Figure Lengend Snippet: TIMP-1 carried by pEVs affects tube formation, but not endothelial migration in vitro . (a) Western Blot and relative quantification showing TIMP-1 overexpression in pEVs. Unpaired T test; * P < 0.05. (b) Analysis of the relative tube lengths in the tube formation assay. The treatment of SVEC4-10 with the anti-TIMP-1 5 µg/mL (AF980 R&D) rescued the tube formation. (c) Conversely, the analysis of relative migration in the scratch assay in the presence of the same anti-TIMP-1 antibody did not shown any restoration of the endothelial migration ability in the presence of pEVs. Data are expressed as means ± SEM (3 independent experiments). Kruskal–Wallis test with Dunn’s multiple comparisons test; * P < 0.05.
Article Snippet: The treatment of SVEC4-10 with the anti-TIMP-1 5 µg/mL (AF980 R&D) rescued the tube formation. (c) Conversely, the analysis of relative migration in the scratch assay in the presence of the same anti-TIMP-1 antibody did not shown any restoration of the endothelial migration ability in the presence of pEVs.
Techniques: Migration, In Vitro, Western Blot, Over Expression, Tube Formation Assay, Wound Healing Assay